Here we prepared static structures of L29W myoglobin to obtain reference structures for our time-resolved crystallographic experiments on that mutant. Panel A shows L29W-Mbmet, panel B L29W-Mbdeoxy and panel C the structure with bound carbon-monoxide. The structure in panel D is obtained by a temperature ramp experiment: A crystal containing molecules with a structure such as the one in panel C was cooled under permanent laser illumination to 100 K. A structure similar to that in panel B was obtained with the CO in the proximal binding site marked D. After increasing the temperature to 180 K, the structure in panel D was determined.