Equipment

Spectrometers

 

  • Bruker Avance III HD™ 500 MHz

    The Bruker Avance III HD™ 500 MHz spectrometer was installed in October 2017.   The system has a 54 mm bore Bruker  Ascend™ superconducting magnet  with active shielding and an  operation field at 11.744 Tesla. The console provides two all digital transmitters and receivers for two RF channels and digital lock. The BLAXH 500/100 amplifier provides 100W power for 1H/19F and 500W for X-nuclei and a GRASP II gradient unit provides 20A for pulsed z-gradients. The BSVT Temperature control unit with BCU II chiller allows variable temperature operation to -40C.

    The instrument is currently used for  structure determination of natural products, drug design and biological systems.  The instrument provides the highest sensitivity for a multitude of one- and two-dimensional experiments (DEPT, APT, COSY, NOESY HSQC, HMBC) and with automatic tune and match capability is the instrument of choice for heteronuclear NMR experiments like 19F, 13C, 15N, 31P and 113Cd.  The instrument is also capable of non linear acquisition and carbon-carbon correlation experiments like ADEQUATE.  While the instrument is primarily used for more advanced experiments and sensitivity limited samples, the sample changer allows the instrument to be used in automated mode by less experienced users, for instructional use in teaching labs and for high throughput analysis.

     

    Probes:

    1. Prodigy™ 5mm X-nuclei optimized double resonance Cryo probe with z-gradientsand Automatic Tune and Match (ATM).  Nuclei: 1H/19F, 31P-15N, Temperature range: -40°C – +80°C
    2. X-nuclei-optimized Broadband probe (Smart™ Probe) with z-gradient and ATM. .   Nuclei:1H, 19F,31P-109Ag Temperature Range: -150°C – +150°C

    Accessories:

    • 24 slot SampleCase™ sample changer
  • Bruker DPX 300 MHz

    The 300 Mhz Spectrometer was installed in 1997. The instrument has an unshielded  7Tesla Bruker superconducting magnet. It is equipped with a BLAXH 100/50 Amplifier for proton/19F  and X-nucleus operation and a GREAT 1/10 gradient amplifier. It continues to be utilized for walk on routine 1D proton and carbon experiments. It is also capable of most  homo and heteronuclear gradient supported 2D experiments (COSY, HSQC, HMBC) which can be performed on a routine basis.

    Probe:

    • 5mm Broadband (BBO) with z-axis gradient. Nuclei: 1H, 31P-183W
  • Oxford Pulsar HF 60 MHz

    pulsar-oxford

    This instrument was installed in 2014 and comes with a 1.4 Tesla benchtop permanent magnet (proton frequency of 60 MHz) and is located on the 4th floor of the teaching wing. It is used by the undergraduate laboratories for proton NMR spectra.

    Probe:

    • 5mm 1H/19F probe

Computers

octaneThe lab is equipped with four Workstations for off line data processing, spectral assignment and structure calculation

 

Workstations

  • marvin.chem.uwm.edu Dell T3500 (Fedora 32/x64)  File and license server  (Name explained)
  • hal.chem.uwm.edu  HP Workstation  (CentOs 7) Web server and backup (Name)
  • vger.chem.uwm.edu Dell Optiplex 745 (Fedora 32/x64)  Workstation for offline processing (Name)
  • nomad.chem.uwm.edu Dell Optiplex 745 (Fedora 32/x64) Workstation for offline processing (Name)
  • deepthought.chem.uwm.edu Dell Optiplex 745 (Fedora 32/x64) Projector (Name)

Printers

  • Dell 5130 Color Laser Printer (located in the NMR facility  B-10)
  • Canon Image Runner ADV 8585/8595 (black and white copier/printer, located in Chemistry-133
  • RICOH MP C3503 PS  (Multi purpose color printer/copier located in Chem-117)

 

Software

Homemade software:

Currently used software includes:

  • BrukerTopspin™ 3.6 Acquisition and processing of 1, 2 or 3D NMR data. Available on the Avance500 and the external Linux workstations. The NMR lab has a five seat network license. The Software can thus be installed and run on personal computers in the department.
  • BrukerTopspin 1.3 Acquisition and processing of 1, 2 or 3D NMR data. Available on DPX300  spectrometer.
  • Mnova  NMR acquisition and processing software is used on the Oxford Pulsar 60 teaching spectrometer.
  • NMR Lab Collection of Useful software (local access only)