Determining Microcystis Genotype Relationships between Lake Winnebago and Green Bay

Tessa Kooij, “Determining Microcystis Genotype Relationships between Lake Winnebago and Green Bay”
Mentor: Ryan Newton, Freshwater Sciences
Poster #108

Harmful algal blooms, particularly those instigated by blooms of Microcystis, a type of cyanobacteria, have become a global concern in freshwater ecosystems, posing threats to water quality and public health due to the production of toxins. Green Bay, a significant recreational area, faces recurrent blooms of this algae, which hinders recreational activities and diminishes the perception of this vital resource. Lake Winnebago, which is connected to Green Bay through the Fox River, also experiences seasonal blooms of cyanobacteria, however, it is unclear whether the species present in Lake Winnebago contribute to the blooms in Green Bay, or if Green Bay harbors its own distinct population of cyanobacteria. This project aims to ascertain if the populations in Lake Winnebago and Green Bay are genetically related. Using techniques to extract DNA and analyzing it with polymerase chain reactions (PCR), gel electrophoresis, and possible DNA sequencing, we will hopefully determine if the genotypes found in Lake Winnebago match those present in Green Bay. The results found will hold implications for informing strategies aimed at mitigating these harmful algal blooms in Green Bay. Ultimately, the results found will help contribute to the decision-making process of treating these waters.